Gruppo guidato da Stefano Geuna
Tel +39 011 670 5433
Fax +39 011 670 5451
Date of birth: Torino, 25-02-1977
Laboratory of Human Anatomy
Department of Clinical and Biological Sciences, University of Turin
Hospital San Luigi, Regione Gonzole 10, 10043 - Orbassano (TO).
December 2011 Researcher at University of Turin
March 2008-2011: Post-doctoral fellowship recipient at University of Turin
November 2007-February 2008: Research Contract at the University of Turin
January 2008: PhD degree in Physiology at University of Turin
July 2002: Degree in Biological Sciences at University of Turin (title of Thesis: Experimental study on peripheral nerve repair by means of muscle-vein-combined grafts)
2000-2002: Internship at Cell Biology Laboratory and Anatomy Laboratory (University of Turin)
2012 – 2014 Teaching of Human Anatomy - Master of Science in Nursing , University of Turin – Italy
2012 – 2014 Teaching of Human Anatomy – Bachelor’s degree course in Motor Sciences, university of Turin, Italy
2012-2014 Teaching of “Peripheral nerve repair with tubulization techniques” at the Reconstructive microsurgery course, Turin, Italy.
2009- 2011 Contract Professor of Human Anatomy – Bachelor’s degree course in Motor Sciences, university of Turin, Italy
2004 – 2011 Teaching Assistant of Human Anatomy – Degree course in Medicine and Surgery, university of Turin, Italy
2005 – 2011 Teaching Assistant of Anatomy Degree in Medical Radiology Techniques for Imaging and Radiotherapy, University of Turin – Cuneo, Italy
2002 - 2011 Teaching Assistant of Anatomy, Nursing school, University of Turin Medical School – Italy
Light, confocal and electron microscopy
Stereological and morpho-quantitative analysis
Retro-transcriptase polymerase-chain-reaction (RT/PCR) and Western Blot
Functional tests for motor recovery (grasping test)
Cell and tissue transplantation
Principal research interests
Peripheral nerve regeneration
Schwann cell biology
Stem cell biology
The scientific activity is principally based on the experimental study of regeneration of peripheral nerve fibers after simple experimental models, like the crush injury, or after the use of innovative surgery techniques, like muscle-in-vein grafts.
Recently my research is focused on the use of adeno-associated virus (AAV) to induce over-expression of angiogenic factors, in particular the vascular endothelial growth factor (VEGF), and gliotrophic factors (Neuregulin), inside a muscle-in-vein graft used o repair a peripheral nerve. The use of AAV is particularly promising because is safer than other approach usually used for the gene therapy, besides it allows to over-express one or more genes in a tissue.
Moreover, since one of the possibility to improve nervous regeneration is the use of cellular transplantation in non-nervous grafts, part of the research is focused on the study of different stem cells, in particular from bone marrow and from adipose tissue.
The analysis of the regeneration is performed by functional test (grasping test), morphological analysis (electron microscopy, histology, immunohistochemistry), stereology, biomolecular (RT-PCR, Western blot).
2008: recipient of a research grant from the Regione Piemonte (Ricerca Sanitaria Finalizzata) for the investigation of peripheral nerve regeneration after gene therapy and tissue engineering.
2008-2009: recipient of a research grant from British Council (Programma Collaborazione Italo-Britannica per giovani ricercatori) for the investigation of peripheral nerve regeneration using an in vitro model of co-culture with adult stem cells and neurons.
2009: recipient of a research grant from the Regione Piemonte (Ricerca Sanitaria Finalizzata) for a pre-clinical study of peripheral nerve regeneration after nerve repair with chitosan membrane and CD34+ stem cells.