F. Benfenati, University of Genova, IIT

Condividi su
Data dell'evento: 22/05/2015
seminars_2015

Friday, 22th May - h 14:00
Seminar room, NICO
Optogenetic Regulation of Transcription: modulation of the RE1-Silencing Transcription Factor (REST) and its possible applications in brain diseases

Fabio Benfenati, Professor of Neurophysiology, University of Genova
Research Director, Department of Neuroscience and Brain Technologies
The Italian Institute of Technology

Fabio_Benfenati

The possibility to manipulate signaling using optogenetic technologies has opened new ways of intervention on cellular function. However, due to the complexity of the mechanisms involved, few attempts have been made so far to modulate transcription of endogenous genes in higher mammals.

The RE1-silencing transcription factor (REST) is a transcriptional repressor controlling several neuronal genes, regulating nervous system development and lineage specification. REST, normally expressed at low levels by mature neurons, it is an important actor of homeostatic plasticity both at excitability and synaptic levels by regulating the transcription of a cluster of neural genes including ion channels and synaptic proteins.

Moreover, REST is dysregulated, more often upregulated in several brain diseases including epilepsy. To interfere with REST dysregulation, we engineered novel optogenetic probes able to spatially and temporally modulate REST action using the LOV2 domain of Avena sativa (AsLOV2), a photosensitive protein that undergoes reversible light-dependent conformational changes.

We generated and validated a series of photoswitchable AsLOV2-peptides able to modulate REST activity when subjected to specific patterns of illumination. These probes provide a way to modulate REST-dependent transcription, and to counteract the long-term changes in gene expression that take place in the pathological environment of the epileptic brain

Host: Alessandro Vercelli

download pdf

Agenda

27 aprile 2018

NICO Progress Report - INN Open Neuroscience Forum

I nostri giovani ricercatori aggiornano i colleghi sulle loro ricerche. Appuntamento ogni due venerdì.

16 febbraio 2019

Torino - 10th International Meeting STEROIDS and NERVOUS SYSTEM

Since 2001, this meeting represented an important event for basic and clinical researchers working on this emerging scientific topic. We will address state-of-the-art approaches in the field of steroids and nervous system, including behavior, epigenetics, genomic and non-genomic actions, the vitamin D, neurodegenerative and psychiatric disorders, and the interference among endocrine disruptors and steroid signaling.

Ricerca

Hervé Barmasse, l'alpinista "figlio del Cervino": perché sostenere i ricercatori del NICO

«Garantire un futuro migliore a chi soffre di malattie che conosciamo bene, come Alzheimer, Sclerosi Multipla e SMA». Il nostro amico Hervé Barmasse spiega perché è importante sostenere il lavoro dei nostri 70 ricercatori. Come? Con una piccola donazione o con il tuo 5xmille. Guarda il video!

11 aprile 2018