Venerdì 28 novembre - ore 11:00
Aula seminari, NICO
Non canonical Drosha pathway regulates hippocampal neural stem cell differentiation
Embryology and Stem Cell Biology, Department of Biomedicine - University of Basel
Self-renewing and multipotent neural stem cells (NSCs) reside in the dentate gyrus of the adult mammalian hippocampus. The Microprocessor, a multimeric complex of the ribonuclease Drosha and the RNA binding protein DGCR8, drives miRNA biogenesis.
The Microprocessor also has miRNA-independent functions, directly targeting and cleaving stem-loop hairpin structures of mRNAs and destabilizing the transcripts. We found that RNAse III Drosha regulates NSC maintenance and inhibits oligodendrocyte fate commitments in adult NSCs.
Drosha-CLIP (cross-linking and immunoprecipitation) experiment shows that Drosha binds the mRNAs of critical oligodendrocyte and gliogenic transcription factors. Taken together our findings reveal a new miRNA-independent action of the Microprocessor in the maintenance of adult NSCs and control of oligodendrocyte differentiation.
Ospite: Annalisa Buffo