Group leader: Carola Eva
Influence of maternal behaviour on the expression of brain plasticity brakes: a role in the susceptibility to anxiety?
2015-2017 | Research Project, University of Torino - Compagnia di San Paolo
Carola Eva co-PI with Daniela Carulli, NICO
The present project aims to unravel a new regulatory mechanism for neuronal plasticity that might underlie a wide range of psychopathologies and to reveal new targets for therapeutic interventions in stress and anxiety-related disorders. We shall combine diverse expertise from neurobiology, glycobiology, soft matter physics and neurophysiology to study if maternal care affects perineuronal nets (PNN) chemical/physical features in the limbic system, thereby influencing neuronal communication within specific networks and, in turn, anxiety. We will further challenge the function of limbic Npy1r mediated signaling in maternal care modulation of structural plasticity.
Complex mechanisms underlying permanent effects of perinatal environment on synaptic plasticity and susceptibilty to psychopathologies: from molecules to tissue properties to behaviour
2014-2015| Fondazione CRT
Carola Eva co-PI with Daniela Carulli and Annalisa Buffo, NICO
This project aims to test if maternal behavior leaves a signature on perineuronal nets (PNN)/myelin assembly, impacting the refinement of circuits involved in anxiety and stress resilience. We shall study if maternal environment affects the chemical and physical features of PNN and myelin development in the limbic system; and if these changes alter neural circuitry, generating anxiety in adults.
We shall also test if PNN modulation or enriched experience will rescue anxiety. Connecting molecular interactions and matrix properties to behaviour, our study will offer mechanistic insight into the role of plasticity modulators in inducing anxiety in early-life stressed mice.
A novel hypothesis on the development of metabolic syndrome in women
2014-2016 | Fondazione Cariplo
The final goals of this proposal are:
- to demonstrate the extent to which ovarian estrogens and liver ERs are responsible for the increased incidence of MetS observed in women after menopause;
- to identify potential mechanisms and the role of brain NPY-Npy1r system in this phenomenon;
- to evaluate the negative or positive effects of specific dietary regimens in females with impaired ovarian functions.
This is a collaborative study that will enable to exploit the most appropriate pharmacological and genetic tools developed in our teams to verify the extent to which specific hepatic factors, as a consequence of ovarian failure and under specific dietary constrains, are able to influence the central nervous system (activity of the NPY-Npy1r system in a mouse model well suited to underline the different vulnerability to MetS of the two sex) and in WAT, liver and muscle. Our study might open the way to the generation of an entire class of novel Estrogen Receptor Modulators to be used for the therapy of MetS.
Effect of substances of abuse, psychoactive drugs, stress and maternal care on brain development and vulnerability to psychopathology
2011-2016 | 2010-2011 PRIN Italian Ministry of University and Research
The goal of this study is to uncover molecular mechanisms through which maternal care permanently affects limbic Npy1r gene expression and its specific contribution to vulnerability to psychopathologies in response to environmental factors, such as acute stress and ethanol exposure. We aim to identify neuronal signaling pathways which interact with limbic Y1R in the regulation of: 1) neuronal plasticity in the limbic regions 2) behavioral, neurochemical and neuroendocrine responses to acute stress and ethanol consumption of mice with low expression of limbic Y1R. Finally, we will investigate whether non-pharmacological or pharmacological treatments may reverse the effects of maternal care on limbic Y1R and related behaviors in adulthood. A greater understanding of these mechanisms may eventually allow specific therapeutic interventions, with a favorable impact on the vulnerability to anxiety disorders and ethanol abuse in adulthood.