O. Butenko, Institute of Experimental Medicine, Czech Republic

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Event date: 01/07/2015
seminars_2015

Thursday, 2nd July - h 14:00
Seminar room, NICO

Expression and functional characterisation of transient receptor potential vanilloid-related channel 4 (TRPV4)   in hippocampal astrocytes after ischemia/reperfusion
Olena Butenko, Department of Cellular Neurophysiology Institute of Experimental Medicine ASCR, v.v.i. Videnska - Czech Republic

During cerebral ischemia a rapid increase of intracellular calcium ([Ca2+]i) initiates dramatic changes in the nervous tissue leading to apoptotic and necrotic cell death and reactive gliosis. Propagating [Ca2+]i waves evoked by ischemia can spread through the astroglial syncytium for a long distance and cause damage in distal CNS regions.

It has been suggested that in astrocytes the massive and uncontrolled plasmalemal Ca2+ entry after hypoxia/ischemia could be mediated by the activation of diverse Ca2+-permeable cation channels and potentially by transient receptor potential vanilloid-related channel 4 (TRPV4).

However, to the best of our knowledge, the role of astrocytic TRPV4 channels during in vivo ischemic injury has not yet been defined. In the first part of the seminar we will discuss pathological changes in the CA1 region of the adult rat hippocampus during the reperfusion after cerebral hypoxia/ischemia (H/I).

The second part of the seminar will be dedicated to the pathophysiological role of TRPV4 channels in adult rat astrocytes – we will talk about the functional expression of TRPV4 channels 1 hour (acute phase of reperfusion) and 7 days (late phase of reperfusion) after H/I in situ as well as in vitro.

Host:  Alessandro Vercelli

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26 february 2020

GLIAL CELLS-NEURON CROSSTALK IN CNS HEALTH AND DISEASE

University of Turin, Italy   
The Workshop is aimed at PhD students and young Postdocs with the goal to promote a thorough understanding of the functions of glial cells in health and disease. The program includes lectures on the newest conceptual advancements and methodological approaches in the study of glial cells in synaptic functions, development and CNS diseases.

Deadline for registration: December 23, 2019.