Development, The Company of Biologists, November 2013
COUP-TFI controls activity-dependent tyrosine hydroxylase expression in adult dopaminergic olfactory bulb interneurons
Serena Bovetti, Sara Bonzano, Donatella Garzotto, Serena Gea Giannelli, Angelo Iannielli
Maria Armentano, Michèle Studer and Silvia De Marchis
COUP-TFI is an orphan nuclear receptor acting as a strong transcriptional regulator in different aspects of forebrain embryonic development. In this study, we investigated COUP-TFI expression and function in the mouse olfactory bulb (OB), a highly plastic telencephalic region in which continuous integration of newly generated inhibitory interneurons occurs throughout life. OB interneurons belong to different populations that originate from distinct progenitor lineages. Here, we show that COUP-TFI is highly expressed in tyrosine hydroxylase (TH)-positive dopaminergic interneurons in the adult OB glomerular layer (GL).
We found that odour deprivation, which is known to downregulate TH expression in the OB, also downregulates COUP-TFI in dopaminergic cells, indicating a possible correlation between TH- and COUP-TFI-activity-dependent action. Moreover, we demonstrate that conditional inactivation of COUP-TFI in the EMX1 lineage results in a significant reduction of both TH and ZIF268 expression in the GL.
Finally, lentiviral vector-mediated COUP-TFI deletion in adult-generated interneurons confirmed that COUP-TFI acts cell-autonomously in the control of TH and ZIF268 expression. These data indicate that COUP-TFI regulates TH expression in OB cells through an activity-dependent mechanism involving ZIF268 induction and strongly argue for a maintenance rather than establishment function of COUP-TFI in dopaminergic commitment.
Our study reveals a previously unknown role for COUP-TFI in the adult brain as a key regulator in the control of sensory-dependent plasticity in olfactory dopaminergic neurons.
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Interferenti endocrini. Il Parlamento europeo impegna la Commissione a emanare norme più restrittive
Buone notizie da Bruxelles. Il Parlamento europeo ha approvato a larga maggioranza una risoluzione che impegna la Commissione (vecchia e nuova) a emanare entro giugno 2020 una normativa più restrittiva che disciplini cosmetici, giocattoli e imballaggi alimentari e che - finalmente - applichi agli EDCs le stesse cautele usate per i composti classificati come sospetti cangerogeni. Una tesi a lungo sostenuta dalla Endrocrine Society e fortemente osteggiata dai rappresentanti della industria chimica.