Giovanni Ferrara, University of Genoa

Share on
Event date: 01/02/2019
seminars_2019

Friday, February 1 st - h 2:00 p.m.
Seminars Room, NICO

Giovanni Ferrara, PhD
Dipartimento di Neuroscienze, riabilitazione, oftalmologia, genetica e scienze materno-infantili (DINOGMI), Università di Genova

Does Nerve-glial antigen 2 (NG2) play a role in dendritic cell activation?

Nerve/glial-antigen 2 (NG2), expressed by oligodendrocyte progenitor cells (OPC), essential for remyelination, and by pericytes, crucial for blood-brain-barrier (BBB) integrity, could be implicated in the pathogenesis of experimental autoimmune encephalomyelitis (EAE). We found that, in addition to macrophages, NG2 is expressed on T cells and dendritic cells (DC) in naïve WT mice.
We described that induction of EAE with myelin oligodendrocyte glycoprotein (MOG35-55) in NG2 knock-out (NG2KO) mice results in a milder EAE than in wild-type (WT) mice with less intense neuropathology associated with improvement in blood-brain barrier. Chimera experiments confirmed the significant role of NG2 expressed by immune cells in EAE: mice reconstituted with NG2KO bone marrow cells develop a milder EAE, regardless of their inherent WT or NG2KO genotype.

Further characterization, demonstrated that, upon MOG35–55-specific recall response of NG2KO T cells in the presence of DCs lacking NG2, is associated with a shift towards a less proinflammatory cytokine profile, with significantly less IFN-γ, but more IL-4 and IL-10. We therefore analyzed MOG-primed lymph node cells for their intracellular expression of IL-12 and we demonstrated that the proportion of IL-12-expressing DCs was significantly lower in NG2KO-affected mice, indicating that NG2KO DCs skew MOG-specific T cell response to an anti-inflammatory Th2 type.
In addition, preliminary data, showed that NG2 is induced upon pro-inflammatory stimulation of DCs. Our data suggest that NG2 plays a role in EAE not only at CNS/BBB level, but also impacting on DC activation and thereby their stimulation of reactive T cells, through controlling IL-12 expression.

Host: Enrica Boda

Events & Meetings

20 september 2019

NICO Progress Report - INN Open Neuroscience Forum 2019

Our young researchers present their work to collegues. From January to December, every two weeks, on friday at 2:00 pm
Seminars Room, NICO

14 november 2019

BraYn 2019 Milan, 14-16 November

The main goal of the BraYn initiative is to organize a scientific conference involving different laboratories across Italy and Europe where young researchers, especially PhD students and junior postdocs, can share their knowledge, skills and ideas to establish new collaborations between different research groups.